Sex of drosophila should be according to the karyotype :-

Sex determination in Drosophila melanogaster depends on Sex Chromosoms,In Drosophila, sex determination is achieved by a balance of female determinants on the X chromosome and male determinants on the autosomes. Normally, flies have either one or two X chromosomes and two sets of autosomes. If there is but one X chromosome in a diploid cell (1X:2A), the fly is male. If there are two X chromosomes in a diploid cell (2X:2A), the fly is female.Thanks