0

Guest

Courses New

a distinct lipo polysaccharide layer is found in gram (+) bacteria gram (-) bacteria all bacteria mycoplasma

a distinct lipo polysaccharide layer is found in
  1. gram (+) bacteria
  2. gram (-) bacteria
  3. all bacteria
  4. mycoplasma

Grade:12th pass

1 Answers

Apoorva Arora IIT Roorkee
askIITians Faculty 181 Points
8 years ago
The correct option is 2.
Gram-negative bacteria are a class of bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation, making positive identification possible. The thin peptidoglycan layer of their cell wall is sandwiched between an inner cell membrane and a bacterial outer membrane. In Gram staining, the outer lipid-based membrane of gram-negative bacteria is removed by an alcohol solution. The alcohol also decolorizes the then exposed peptidoglycan layer by dissolving away the previously applied crystal violet. A counterstain (safranin or fuchsine) is then added which recolorizes the bacteria red or pink.

Gram-positive bacteria on the other hand have a thicker peptidoglycan layer in their cell wall outside the cell membrane, which retains the crystal violet stain during the alcohol wash, so long as it is timed correctly. The counter stain may also be absorbed by gram positive bacteria but the darker crystal violet stain predominates visually.

Think You Can Provide A Better Answer ?

Provide a better Answer & Earn Cool Goodies See our forum point policy

Other Related Questions on Botany

View all Questions »

ASK QUESTION

Get your questions answered by the expert for free

Answer and earn gift vouchers

Win Gift vouchers upto Rs 500/-

View courses by askIITians

Design classes One-on-One in your own way with Top IITians/Medical Professionals

Click Here Know More

Complete Self Study Package designed by Industry Leading Experts

Click Here Know More

Live 1-1 coding classes to unleash the Creator in your Child

Click Here Know More

a Complete All-in-One Study package Fully Loaded inside a Tablet!

Click Here Know More