Question icon
Grade 12Organic Chemistry

expalin me about Benedict’s test andSchiff’s Base test

Profile image of KALYAN
10 Years agoGrade 12
Answers icon

3 Answers

Profile image of anil kumar
10 Years ago

The Schiff test is an early organic chemistry name reaction developed by Hugo Schiff,[1] and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues.[2] The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfitepararosaniline (which lacks an aromatic methyl group) and new fuchsin (which is uniformly mono-methylated ortho to the dye's amine functionalities) are dye alternatives with comparable detection chemistry.

In its use as a qualitative test for aldehydes, the unknown sample is added to the decolorized Schiff reagent; when aldehyde is present a characteristic magenta color develops. Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain andperiodic acid-Schiff stain. Human skin also contains aldehyde functional groups in the termini of saccharides and so is stained as well.

Profile image of SAI SARDAR
10 Years ago
Dear Kalyan,
                     Benedict’s  Test for non-reducing Sugars is a test which determines the presence of non-reducing sugars in a test solution.
Profile image of N JYOTHEESWAR
10 Years ago
hai kalyan,
             benedicts test is used for decting glucose in urine in which urine is heated with a blue solution containing sodium carbonate,sodium citrate................
     schiff test is used for detection of many organic aldehydes that has also found use in staining of biological tissues.