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Which ONE of the following combinations of molecular masses of polypeptides are obtained from purified human IgM when analysed on sodium dodecyl suplhate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions? 55 kDa, 15 kDa (B) 70 kDa, 25 kDa, 15 kDa (C) 55 kDa, 25 kDa (D) 155 kDa

Which ONE of the following combinations of molecular masses of polypeptides are obtained from purified human IgM when analysed on sodium dodecyl suplhate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions?
  1. 55 kDa, 15 kDa (B) 70 kDa, 25 kDa, 15 kDa (C) 55 kDa, 25 kDa (D) 155 kDa

Grade:12

1 Answers

Arun
25750 Points
5 years ago
IgM is the major antibody produced in the primary immune response and it exists as part of the B-cell antigen receptor on the surface of B cells, and as a secreted glycoprotein. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is used to obtain the combinations of molecular masses of polypeptides from purified human IgM. The combinations obtained from SDS-PAGE are 70kDa, 25kDa and 15kDa.
So, the answer is B.

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