A multi subunit enzyme runs at 1000kDa in native PAGE and shows three bands of mol.wt 30kDa, 20 kDa, and 10kDa in sds page. The enzyme is more likely to contain the following number of subunits. A)3 B)4 c)7 D)8
Nivedita Rao , 7 Years ago
Grade 12th pass
2 Answers
Dr.Adam Shaik
Last Activity: 7 Years ago
First of all by using the SDS-PAGE technique Dissociation of protein sub units is possible. The question is wrong In the given question 1000kDa is given But if we consider all “8” sub units are of 30kDa its only 240kDa and not1000kDa.
When these type of questions are asked: Just remember that Sum (addition) of all subunits must give the total proetin weight in kDa.
Ajeet singh
Last Activity: 7 Years ago
I agree with the answer. We can not calculate the total number of subunits with given data but type of subunits can be answered. As there are three different SDS PAGE band, so three type subunit will be there. But we can not tell the total number of subunits.
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