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i want know how to detect Urea in fish meal

what is method to detrmine urea%


6 years ago


Answers : (1)


it is possible to determine the urea concentration in urine using the enzyme urease which catalyses the following reaction:

Urea+Water --> (with urease on top of arrow) --> Ammonium Carbonate

Ammonium carbonate has an alkaline ph.

Urease breaks down urea, the pH of the reaction mixture changes. Change can be shown by pH indicator. If reaction goes to completion the concentration of ammonium carbonate can be found by titrating against 0.1 mol dm-3 HCL. Volume of HCL used is an estimate of concentration of urea in original solution.




6 years ago

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