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1. A notochord is present at some stage in the life of a chordate.
2. Central nervous system is dorsal and hollow.
3. Gill slits are present in the pharynx either in the embryo or adult.
4. Tail is present at some stage in the life of the chordate.
5. Heart is ventral.
6. If present RBCs contain respiratory pigment (haemoglobin).
1. Notochord is not present at any stage in the life of a non-chordate.
2. Central nervous system is ventral and solid.
3. Gill slits are absent.
4. Tail is absent.
5. Heart is dorsal (if present).
6. If haemoglobin or other respiratory pigment is present, it is found in the blood plasma. RBCs are absent.
Chordata
1. Notochord is present in embriyo.
2. Central dorsal, hollow nerve is present.
3. Phayregial gill slits present.
4. Heart non vertebral.
5. Hepatic portal system present.
6. RBC posses hemoglobin.
7. Blood flows in dorsal vessel from before backwards.
Non-Chordta
1. Notochord is absent
2. Central nervous system
3. Pharyngeal gill slit is absent.
4. Heart dorsal.
5. Hepatic portal system absent.
6. If present dissolve in plasma.
7. Blood flows in dorsal vessel from behind forward.
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