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Grade: 10
        
What is absorption spectroscopy ??????????????????????????????????????? ??? 
9 months ago

Answers : (3)

Shivangi Khatter
askIITians Faculty
468 Points
							dear student
absorption spectroscopy is technique used to determine concentration and structure of a sample based on which wavelengths of liquid are absorbed.
9 months ago
Arun
22545 Points
							
Absorption spectroscopy is one of the most widely used techniques employed for determining the concentrations of absorbing species (chromophores) in solutions. It is a nondestructive technique which biologists and biochemists and now systems biologists use to quantify the cellular components and characteristic parameters of functional molecules. This quantification is most relevant in the context of systems biology. For creating a quantitative depiction of a metabolic pathway, a number of parameters and variables are important and these need to be determined experimentally. This chapter describes the UV–visible absorption spectroscopyused to produce experimental data for bottom-up modeling approaches of systems biology which uses concentrations and kinetic parameters (Km and Vmax) of enzymes of metabolic/signaling pathways, intracellular concentrations of metabolites and fluxes. It also briefly describes the application of this technique for quantification of biomolecules and investigating biomolecular interactions.
9 months ago
Varsha
18 Points
							
Absorption spectroscopy is a molecular spectroscopy method that uses the wavelength dependent on absorption  characteristics of materials to identify and quantify the specific substances. The infrared and ultra violet visible spectroscopy  is particularly common in this type of analytical applications. 
9 months ago
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