what does "absorbance of the solution" mean?

Dear Student

The absorbance A of a solution is defined as:

A=log₁₀(I₀/I)

In which I₀ is the incident light intensity, and I is the transmitted light intensity. The absorbace is related to the molar absorption coefficient(extinction coefficient) ε (in M^-1 cm^-1), concentration c (in M) and path length l (in cm) by

A= εlc

The absorption coefficient of myoglobin at 580nm is 15,000 M^-1 cm^-1.

The Greek letter epsilon in these equations is called the molar absorptivity - or sometimes the molar absorption coefficient.

All the best.                                                           

AKASH GOYAL

AskiitiansExpert-IIT Delhi

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