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ragha vendra Grade: 12
        what does "absorbance of the solution" mean?
7 years ago

Answers : (1)

AKASH GOYAL AskiitiansExpert-IITD
419 Points

Dear Student

The absorbance A of a solution is defined as:


In which I0 is the incident light intensity, and I is the transmitted light intensity. The absorbace is related to the molar absorption coefficient(extinction coefficient) ε (in M-1 cm-1), concentration c (in M) and path length l (in cm) by

A= εlc

The absorption coefficient of myoglobin at 580nm is 15,000 M-1 cm-1.

The Greek letter epsilon in these equations is called the molar absorptivity - or sometimes the molar absorption coefficient.


All the best.                                                           


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7 years ago
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