Column chromatography is a chromatography technique used to separate mixture of chemical substances into its individual compounds. Column chromatography is a widely used method for the purification or separation of chemical compound mixture in lab.
Column Chromatography consists of two phases: one mobile phase and one contiguous stationery phase. The stationery phase is solid and the mobile phase is liquid. The compound mixture moves along with the mobile phase through stationery phase and separates depending on the different degree of adhesion (to the silica) of each component in the sample or the compound mixture.
We can purify a mixture containing eicosane, pentanoic acid, ethyl butanoate, and 2 bromohexane on making different mobile phase.
