Thank you for registering.

One of our academic counsellors will contact you within 1 working day.

Please check your email for login details.
MY CART (5)

Use Coupon: CART20 and get 20% off on all online Study Material

ITEM
DETAILS
MRP
DISCOUNT
FINAL PRICE
Total Price: Rs.

There are no items in this cart.
Continue Shopping

You went to the field to collect a sample and digested it, how can you determine the presence of sodium and potassium present in your sample?

You went to the field to collect a sample and digested it, how can you determine the presence of sodium and potassium present in your sample?

Grade:

1 Answers

Aman Bansal
592 Points
8 years ago

Dear Anumah,

The unusual hypotonicity of equine blastocyst fluid hasprompted us to investigate the role of sodium- and potassiumdependentadenosine triphosphatase (Na+,K+-ATPase) in theprocess of fluid accumulation in the horse conceptus. Ninemares were used for the experiments. Reverse transcriptasepolymerase chain reaction was conducted on two sets of fiveconceptuses recovered between 12 and 28 days (+ 1 day) afterovulation. Messenger RNAs encoding the ct1 and pi subunitisoforms of Na+,K+-ATPase were detected in all embryonic tissuesexamined. Western blot analysis showed that oal and 31subunits are both present in Day 15 conceptuses. Trophoblasttissues from 19 conceptuses between 8 and 31 days after ovulationwere stained immunohistochemically using primary antibodiesagainst the aol and 31 subunit isoforms of the Na+,K +-ATPase. Both isoforms were detected in all sections. Trophoblasticvesicles, prepared from 6 conceptuses between 12 and14 days after ovulation, were used to investigate the inhibitionof blastocyst expansion with ouabain after collapse induced withcytochalasin D. In normal medium there was a mean 3-fold increase,and in ouabain (10-6 M) a mean 3-fold decrease, in thevolume of vesicles that had been partially collapsed with cytochalasinD. We therefore conclude that, despite the hypotonicityof the blastocyst fluid in the early horse conceptus, the Na+,K +-ATPase plays a role in its accumulation, as in other species.

Cracking IIT just got more exciting,It s not just all about getting assistance from IITians, alongside Target Achievement and Rewards play an important role. ASKIITIANS has it all for you, wherein you get assistance only from IITians for your preparation and win by answering queries in the discussion forums. Reward points 5 + 15 for all those who upload their pic and download the ASKIITIANS Toolbar, just a simple  to download the toolbar….

So start the brain storming…. become a leader with Elite Expert League ASKIITIANS

Thanks

Aman Bansal

Askiitian Expert

Think You Can Provide A Better Answer ?

Provide a better Answer & Earn Cool Goodies See our forum point policy

ASK QUESTION

Get your questions answered by the expert for free