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The main features of crossing over are given below:
1. Crossing over takes place during meiotic prophase, i.e., during pachytene. Each pair of chromosome has four chromatids at that time.
2. Crossing over occurs between non-sister chromatids. Thus one chromatid from each of the two homologus chromosomes is involved in crossing over.
3. It is universally accepted that crossing over takes place at four strand stage.
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