Where does ATP synthesis occur in mitochondria?

A

Outer mitochondrial membrane

B

Inner mitochondrial membrane

C

Intercristal space

D

Intermembrane space

ATP synthesis occurs in the inner mitochondrial membrane in mitochondria. The enzyme required for the synthesis of ATP is ATP synthase. It is located in the inner mitochondrial membrane. There is transfer of protons from the matrix to the inner mitochondrial membrane. This creates a concentration gradient of protons. ATP synthase uses this concentration gradient to synthesize the energy rich molecule ATP.