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When genomic DNA is fragmented and cloned, the screening of the desired gene is done by using

When genomic DNA is fragmented and cloned, the screening of the desired gene is done by using

Grade:12th pass

2 Answers

Aabid Hussain
askIITians Faculty 576 Points
6 years ago
Hi,

It depends upon the size of DNA fragment or the length of the nucleotide obtained. However there are some basic methods through which the gene of intrest can be screened out:

i) Nucleic acid hybridizations
ii) Antibody probes
iii) Polymerase Chain Reaction(PCR)
iv) Restriction Fragment analysis
v) DNA sequencing
raj
384 Points
6 years ago
hello,a. Nucleic acid hybridizationsb. Antibody probesc. Polymerase Chain Reaction(PCR)d. Restriction Fragment analysise. DNA sequencing

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