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What is Recombinant DNA? How do enzyme restriction endonuclease and DNA ligase help its formation?

What is Recombinant DNA? How do enzyme restriction endonuclease and DNA ligase help its formation?

Grade:12

1 Answers

Simran Vinaik
askIITians Faculty 1230 Points
5 years ago
Recombinant DNA is Vector DNA joined with Gene of interest. DNA from a donor organism or biological source is first extracted from cells and then subjected to a cutting process known as enzymatic restriction using RESTRICTION ENZYME. This generates fragments of DNA that contain the gene or genes of interest. They are next inserted into larger DNA molecules ("plasmids") using DNA LIGASE ( acts like molecular glue) which are placed into a bacteria and allowed to multiply.554-813_recombinant DNA.jpg554-84_Rdna.jpg

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