The correct option is 1.
Protoplast, from ancient Greek πρωτόπλαστος (prōtóplastos, "first-formed"), initially referred to the first human or, more generally, to the first organized body of a species. In modern biology, it has several definitions:
A protoplast is a plant, bacterial or fungal cell that had its cell wall completely or partially removed using either mechanical or enzymatic means.
Protoplasts: Have their cell wall entirely removed and are derived from gram + (gram-positive)
Spheroplasts: Have their cell wall only partially removed and are gram - (gram-negative)
More generally protoplast refers to that unit of biology which is composed of a cell's nucleus and the surrounding protoplasmic materials.
