Protoplasts of two different cells can be made to fuse with the help of Polyethylene glycol(PEG) Calcium chloride Sodium bicarbonate CHI

The correct option is 1.
Protoplast, from ancient Greek πρωτόπλαστος (prōtóplastos, "first-formed"), initially referred to the first human or, more generally, to the first organized body of a species. In modern biology, it has several definitions:

A protoplast is a plant, bacterial or fungal cell that had its cell wall completely or partially removed using either mechanical or enzymatic means.
Protoplasts: Have their cell wall entirely removed and are derived from gram + (gram-positive)
Spheroplasts: Have their cell wall only partially removed and are gram - (gram-negative)
More generally protoplast refers to that unit of biology which is composed of a cell's nucleus and the surrounding protoplasmic materials.