Perfect homozygous individuals are produced by

1. Meristem culture
2. Anther culture
3. Protoplast culture
4. Somatic hybridization

The correct option is 2.
The technique was discovered in 1964 by Guha and Maheshwari. This technique can be used in over 200 species, including tomato, rice, tobacco, barley, and geranium. Some of the advantages which make this a valuable method for obtaining haploid plants are:
the technique is fairly simple
it is easy to induce cell division in the immature pollen cells in some species
a large proportion of the anthers used in culture respond (induction frequency is high)
haploids can be produced in large numbers very quickly.