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NEET UG 1 Q126 paper code x . Have confusion between zinc finger analysis and DNA-DNA hybridisation..

NEET UG 1 Q126 paper code x . Have confusion between zinc finger analysis and DNA-DNA hybridisation..

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Grade:12th pass

1 Answers

Aabid Hussain
askIITians Faculty 576 Points
7 years ago
Hi Anmol,
Thank you for asking

First of all i will make you clear about what is zinc finger.
Zinc finger is the structural motif(region) where you will find coordination among one or more zinc ions in order to stabilize the protein fold. Zinc finger analysis is nothing to do with DNA fingerprinting steps.

Now coming to your next confusion about DNA-DNA hybridization.
DNA-DNA hybridization is one of the event of PCR(Polymerase Chain Reaction).

Here i’m providing the step wise representation of DNA fingerprinting

1. DNA sampling (collection of DNA sample)
2. Formation of RFLP(Restriction digestion length polymorphism) by Restriction enzymes
3. Polymerase chain reaction (to amplify the RFLP). During this phase DNA-DNA hybridization occurs.
4. Autoradiography (Development of DNA pattern in the form of varios bands)

Hope the answer of the question.

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