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Grade 12th passBotany

How would fingerprints on a cuvette during a visible spectrometry lab affect the concentration reading?
A
Concentration would be lower, since more light is being reflected and not detected by the spectrometry light sensor.
B
Concentration would be higher since more light is being reflected or absorbed, increasing the absorbance reading.
C
Concentration will stay the same since the fingerprints will only deflect the light slightly and not enough to affect the absorbance measurements.
D
Concentration will be increase since more light is absorbed by the solution, resulting in a decrease in the absorbance measurement.

Profile image of ankit singh
5 Years agoGrade 12th pass
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1 Answer

Profile image of Rituraj Tiwari
5 Years ago
Fingerprints on a cuvette would affect absorbance and concentration calculation by the fact that fingerprints absorb and scatter light slightly, despite not be readily visible. A cuvette (with fingerprints on it) will give a slightly higher absorbance reading and the measured concentration will be significantly higher than the actual concentration.Hence the required answer is- (B)Concentration would be higher since more light is being reflected or absorbed, increasing the absorbance reading.