Raheema Javed
Last Activity: 9 Years ago
The advantages of usingE. colias the host organism are well known.
(i) It has unparalleled fast growth kinetics. In glucose-salts media and given the optimal environmental conditions, its doubling time is about 20 min. This means that a culture inoculated with a 1/100 dilution of a saturated starter culture may reach stationary phase in a few hours.
(ii) High cell density cultures are easily achieved.
(iii) Rich complex media can be made from readily available and inexpensive components.
(iv) Transformation with exogenous DNA is fast and easy. Plasmid transformation ofE. colican be performed in as little as 5 min