Gene library can be constructed by

A) reverse transcriptase method

B) shot gun

C) chemical synthesis method

D) DNA-RNA hybridization method...

Please answer this question with reason...

Thankyou

Ans is shotgun sequencing wherethe DNA is subjected to random cleavage by restriction endonucleases.Partial digestion allows the formation of majority of DNA fragments with a length of 10-30 kb. The fragments are frequently overlapping and they can be fractionated by gel electrophoresis. The isolated fragments (approximately 20 kb in size) are inserted into λ phage vector and cloned.