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Gene library can be constructed by A) reverse transcriptase method B) shot gun C) chemical synthesis method D) DNA-RNA hybridization method... Please answer this question with reason... Thankyou

Gene library can be constructed by
A) reverse transcriptase method
B) shot gun
C) chemical synthesis method
D) DNA-RNA hybridization method...
Please answer this question with reason...
Thankyou

Grade:12th pass

1 Answers

Simran Vinaik
askIITians Faculty 1231 Points
2 years ago
Ans is shotgun sequencing wherethe DNA is subjected to random cleavage by restriction endonucleases.Partial digestion allows the formation of majority of DNA fragments with a length of 10-30 kb. The fragments are frequently overlapping and they can be fractionated by gel electrophoresis. The isolated fragments (approximately 20 kb in size) are inserted into λ phage vector and cloned.554-1998_ss.jpg

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