0

Guest

Courses New

Gene library can be constructed by A) reverse transcriptase method B) shot gun C) chemical synthesis method D) DNA-RNA hybridization method... Please answer this question with reason... Thankyou

Gene library can be constructed by
A) reverse transcriptase method
B) shot gun
C) chemical synthesis method
D) DNA-RNA hybridization method...
Please answer this question with reason...
Thankyou

Grade:12th pass

1 Answers

Simran Vinaik
askIITians Faculty 1230 Points
5 years ago
Ans is shotgun sequencing wherethe DNA is subjected to random cleavage by restriction endonucleases.Partial digestion allows the formation of majority of DNA fragments with a length of 10-30 kb. The fragments are frequently overlapping and they can be fractionated by gel electrophoresis. The isolated fragments (approximately 20 kb in size) are inserted into λ phage vector and cloned.554-1998_ss.jpg

Think You Can Provide A Better Answer ?

Other Related Questions on Botany

View all Questions »

ASK QUESTION

Get your questions answered by the expert for free

Answer and earn gift vouchers

Win Gift vouchers upto Rs 500/-

View courses by askIITians

Design classes One-on-One in your own way with Top IITians/Medical Professionals

Click Here Know More

Complete Self Study Package designed by Industry Leading Experts

Click Here Know More

Live 1-1 coding classes to unleash the Creator in your Child

Click Here Know More

a Complete All-in-One Study package Fully Loaded inside a Tablet!

Click Here Know More