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Cybrid is a result of Fusion of cytoplasm and ruclei of the two somatic cells Fusion of cytoplasm of two somatic cell but the nuclei remain unfused Fusion of cytoplasm of two somatic cells occurs bvut the nucleus of other degenerates Fusion of cytoplasm of two somatic cells takes place but one part of the nucleus of one cell fuses with the entire nucleus of second cell

Cybrid is a result of 
  1. Fusion of cytoplasm and ruclei of the two somatic cells
  2. Fusion of cytoplasm of two somatic cell but the nuclei remain unfused
  3. Fusion of cytoplasm of two somatic cells occurs bvut the nucleus of other degenerates
  4. Fusion of cytoplasm of two somatic cells takes place but one part of the nucleus of one cell fuses with the entire nucleus of second cell
  5.  

Grade:12th pass

1 Answers

Apoorva Arora IIT Roorkee
askIITians Faculty 181 Points
6 years ago
The correct option is 3.
A cytoplasmic hybrid (or cybrid, a portmanteau of the two words) is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast. Cytoplasts are enucleated cells. This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the fusion. Rho-zero cells are cells which have been depleted of their own mitochondrial DNA by prolonged incubation with ethidium bromide, a chemical which inhibits mitochondrial DNA replication. The rho-zero cells do retain mitochondria and can grow in rich culture medium with certain supplements. They do retain their own nuclear genome. A cybrid is then a hybrid cell which mixes the nuclear genes from one cell with the mitochondrial genes from another cell. Using this powerful tool, it makes it possible to dissociate contribution from the mitochondrial genes vs that of the nuclear genes.

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