Difference between light microscope and electron microscope ......
Divyanshu Darshan , 7 Years ago
Grade 12
9 grade science> Difference between light microscope and e...
3 Answers
Priyanshu priyadarshi
Last Activity: 7 Years ago
Light microscope uses glass lens whereas electron microscope uses electromagnets......... ............... ................
Arun
Last Activity: 7 Years ago
| Light Microscope | Electron Microscope (EM) | |
| 1 | Techniques are simple | High level of technical skill needed |
| 2 | Specimen preparation normally takes a few minutes to few hours | Specimen preparation often takes several days |
| 3 | Live or dead specimens may be seen | Only dead and dried specimens can be seen |
| 4 | Surface view of the specimen is poor | Good surface details (SEM) or internal details (TEM) can be seen. |
| 5 | Light rays are used to illuminate the specimen. | Electron beam produced by the cathode is used |
| 6 | Condenser, objective and eye piece lenses are made of glass. | All lenses are electromagnets |
Scholr Boy
Last Activity: 6 Years ago
| Light microscope | Electron microscope |
| Simple to use | Users require technical skills |
| Can view both live and dead specimens | Views only dead specimens |
| Poor surface view | Good surface view and internal details |
| Uses light rays to illuminate specimens | Uses a beam of electrons to view specimens |
| Lenses are made of glass | Lenses are made of electromagnets |
| Low resolving power, usually below 0.30µm. | High resolving power of up to 0.0001µm. |
| Low magnification of up to 1,500x | High magnification of up to 1,000,000x |
| Images are viewed by the eyes through the eyepiece | Images are viewed on a photographic plate or zinc sulphate fluorescent screen |
| Not used under a vacuum | Operates under a high vacuum |
| Cheap to buy and has low maintenance costs | Very expensive to buy and maintain |
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