Fructose and Glucose can be distinguished by(A) Fehling’s test(B) Barfoed’s test(C) Benedict’s test(D) Seliwanoff’s test

The correct answer is (D) Seliwanoff's test.

Seliwanoff's test is used to differentiate between fructose and glucose, which are both monosaccharides. In this test, a sample containing the sugar is heated in the presence of a resorcinol reagent and hydrochloric acid. Fructose, being a ketose sugar, reacts more rapidly with the reagent compared to glucose, which is an aldose sugar. As a result, fructose produces a red color, while glucose produces a less intense color or no color change at all.

Fehling's test, Barfoed's test, and Benedict's test are also commonly used to distinguish between reducing sugars, including fructose and glucose, but they are not specific to differentiating fructose from glucose. These tests rely on the ability of reducing sugars to reduce certain metal ions, such as copper, resulting in the formation of a colored precipitate. The differences in the rates of reaction and the colors obtained can provide information about the presence of reducing sugars but do not specifically distinguish between fructose and glucose.