Phase of shortest duration is:

• A. Prophase
• B. Metaphase
• C. Anaphase
• D. S-phase

The phase of shortest duration in the cell cycle is typically Metaphase. During this stage, chromosomes align at the cell's equatorial plane, and this alignment is crucial for proper separation during the next phase.

Understanding the Cell Cycle Phases

Here’s a brief overview of the phases mentioned:

• Prophase: Chromosomes condense and become visible, and the nuclear envelope begins to break down.
• Metaphase: Chromosomes line up in the center of the cell, making it the shortest phase.
• Anaphase: Sister chromatids are pulled apart to opposite sides of the cell.
• S-phase: This is part of interphase where DNA is replicated, taking a longer duration.

In summary, while all phases are important for cell division, Metaphase is the quickest, ensuring that chromosomes are properly positioned for separation.