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Respiration in Plants

What is Plant Respiration

“Plant respiration is the chemical reaction by which plants cells stay alive.” The process of respiration is expressed as: 

Glucose + Oxygen → Carbon Dioxide + Water (+ Energy)


Do Plants Breathe?

The answer to this question is not direct. Yes, plants need oxygen for respiration but at the same time they also give out carbon dioxide. Thus, plants have proper system to ensure the availability of oxygen. Unlike animals, plants do not possess any specialized organs for exchange of gases but they have lenticels and stomata (present in stems and leaves respectively) that carry out the function of gaseous exchange.

Plants do not have any specialized organ to respire and exchange gases because each part of the plant takes care of the need of gases themselves. The parts of the plant do not display any great demand for exchange of gases. Added to this, stems, leaves and roots respire at very lower rate as compared to animals. But during the process of photosynthesis, large exchange of gases takes place and each part of the plant is well adapted to fulfill its need of gases. Availability of oxygen is not a problem during photosynthesis because the cells release oxygen within cells. It is important to note that each living cell in a plant is located quite close to the surface of the plant and in case of stems, the living cells are arranged in the form of thin layers beneath and inside the bark and have openings which are referred as lenticels. Thereby, the respiration and translocation takes place at every part of the plant.

The complete combustion of glucose produces H2O and CO2 as end products and release energy in the form of heat. In case, this energy is required by the cell, it will utilize accordingly. Following reaction explains the entire process:

C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy

During the process of respiration, O2 is utilized and carbon dioxide, energy and water are released as products. There is also a situation when then the oxygen is not available. For instance, the first cell on this planet must have carried out reaction in the absence of oxygen and even in the current living world we are aware of several living organisms adapted to anaerobic conditions. Some of these organisms are facultative and some are obligate. In any of these cases, all living organisms retain enzymatic machinery to partially oxidize glucose in the absence of oxygen. This process is also called as Glycolysis which includes breaking down of glucose to Pyruvic Acid

Respiration In Roots

The process of respiration in roots is carried out in the following manner: 

  • Air occurs in several interspaces of soil. The hairs of the roots are in direct contact with them.

  • Oxygen of the soil gets diffused via root hairs and reaches all internal cells of the root for respiration.

  • Carbon dioxide produced during the diffusion is released in the opposite direction.

  • In the condition of water logging, air gets deficient in soil and in this case, metabolic activity of the roots declines.

Respiration In Stems

  • The stems of herbaceous plants possess stomata and the air gets diffused via it and reaches the cells for respiration.

  • The carbon dioxide produced during the process gets diffused in the air via stomata.

  • When the stems are woody, this gaseous exchange is carried out by lenticels.

Respiration In Leaves

  • Leaves of the plants have tiny pores which are referred as stomata. The exchange of gases takes place by the process of diffusion via stomata. The stomata are present in large number on lower surface of leaves of plant. Each stoma is surrounded and controlled by Guard Cells (two kidney shaped cells). Then the stoma, open gaseous exchange takes place between Atmosphere and Interior of Leaves.

Types of Respiration

Respiration is of two types

  • Aerobic Respiration: In this type of respiration, the food substances are completely oxidized into H2O and CO2 with the release of energy. It requires atmospheric oxygen and all higher organisms respire aerobically. Following figure shows the steps included in Aerobic Respiration.

Aerobic Respiration

  •  Anaerobic Respiration: In this type of respiration, partial oxidation of food takes place and energy is released in the absence of oxygen. This type of respiration occurs in prokaryotic organisms like bacteria and yeast. Ethyl alcohol and carbon dioxide are formed in this process.


The term glycolysis is derived from two Greek words, i.e. Glycos which means sugar and lysis means splitting. The scheme of glycolysis was given by Otto Meyerhof, J. Parnas and Gustav. In case of anaerobic respiration, respiration is carried out via glycolysis which occurs in cytoplasm of the cells. In it, partial oxidation of glucose is carried out resulting in two molecules of pyruvic acid. Glucose and fructose are phosphorylated to give rise to glucose-6-phosphate via enzyme hexokinase. This phosphorylated glucose is isomerized to produce fructose-6-phosphate. 

The several steps of Glycolysis are depicted in the figure below. In this process, chain of ten reactions takes place under the control of various enzymes and the outcome is pyruvate. ATP is utilized at two steps:

  • During the conversion of glucose into glucose-6-phosphate.

  • During the conversion of fructose-6-phosphate in fructose 1 and 6-diphosphate.

The fructose 1, 6 diphosphate is broken into

(i). Dihydroxyacetone Phosphate and

(ii). 3-Phosphoglyceraldehyde (PGAL). 




FermentationIn this process, the incomplete oxidation of glucose is carried out under anaerobic conditions via set of reactions where pyruvic acid is converted into ethanol and carbon dioxide. These reactions are catalyzed by two enzymes, i.e. achohol dehydrogenase and acid-decarboxylase. Other organisms such as bacteria produce lactic acid from pyruvic acid. The detailed steps are depicted in the figure below. In animal cells as well, during muscle exercise, in case of inadequacy of oxygen for cellular respiration, pyruvic acid is reduced to lactic acid by lactate dehydrogenase. NADH+H+ are the reducing agent which is oxidized to NAD+ in the process.

In both alcohol and lactic acid fermentation, very less energy is released. Both these process are hazardous because alcohol or acid is produced during the process. Fermentation process is used in our daily life such as in the formation of curd, vinegar, bread and alcoholic drinks.


Aerobic Respiration

For aerobic respiration to take place in mitochondria, pyruvate is transported into mitochondria from cytoplasm. The most important events in this respiration are: 

  • The hydrogen atoms, that leaves 3 molecules of CO2.
  • Passing on of electrons removed as a part of hydrogen atoms to molecular oxygen with simultaneous synthesis of Adenosine Triphosphate (ATP).

Pyruvate, formed during glycolytic catabolism of carbohydrates in cytosol, enters the matrix of mitochondria and it undergoes oxidative decarboxylation by the complex set of reaction. This entire process is catalyzed by pyruvic dehydrogenase and this reaction requires involvement of several coenzymes such as Coenzyme A and NAD+.


During this entire process, 2 molecules of NADH are produced from the metabolism of 2 molecules of pyruvic acid. The acetyl CoA enters into a cyclic pathway called as Kreb’s cycle or tricarboxylic acid. The name Krebs Cycle is mentioned after the name of scientist Hans Kreb who first elucidated this cycle.

Tricarboxylic Acid Cycle

It is the second stage of cellular respiration. It plays an integral role in catabolism of breaking down of organic fuel molecule i.e. glucose, sugar, fatty acid and amino acids. The cycle starts with the condensation of acetyl group with oxaloacetic acid and water to release citric acid. The overall reaction of Krebs cycle is – 

2 acetyl CoA + 6NAD+ + 2FAD + 2ADP + 2P→ 4 CO2 + 6 NADH + 6H+ + 2 FADH2 + 2ATP

This reaction is catalyzed by citrate synthase enzyme and a molecule of CoA is released. This citrate is then isomerized to isocitrate followed by decarboxylation that results in the formation of α-ketoglutaric acid and succinyl-CoA. Then succinyl-CoA is oxidized to OAA allowing the cycle to continue. During this conversion of succinyl-CoA to succinic acid one molecule of GTP is synthesized. In a coupled reaction GTP is converted to GDP along with the synthesis of ATP from ADP. Added to this, at three places in the entire cycle, NAD+ is reduced to NADH + H+ and at one point FAD+ is reduced to FADH2. The entire cycle is shown in the figure below :

Tricarboxylic Acid Cycle

Furthermore, the continued oxidation of acetic ued oxidacid in this cycle requires continued replenishment of oxaloacetic acid, i.e. the first member of the cycle. The summary equation of entire process is given below -


Electron Transport System (ETS) and Oxidative Phosphorylation

Electron Transport System (ETS) and Oxidative PhosphorylationNADH and FADH2 carry electrons to the Electron Transport System. After the completion of Krebs cycle, oxygen enters in pathway as the electron acceptor at the end of electron transport system. “The metabolic pathway, through which the electron passes from one carrier to another, is called electron transport system and is present in the inner mitochondrial membrane.” The electrons produced from NADH in the matrix of mitochondria during krebs/ citric acid cycle are oxidized by an NADH dehydrogenase (Complex I). The electrons are then transported to ubiquinone present in the inner membrane. This ubiquinone also receives reducing equivalents by FADH2 (Complex II). This FADH2 is generated during the oxidation of succinate in Krebs cycle. This reduced ubiquinone is oxidized with the transfer of electrons to cytochrome c with cytochrome bc1 complex (Complex III). The small protein cytochrome c attached to outer surface of inner membrane acts as mobile carrier that transfers the electrons from Complex III to Complex IV. This Complex IV is cytochrome coxidase complex which contains cytochrome a and a3, along with two copper centers.

When the transference of electron takes place from one carrier to another via complex I to IV, they are coupled to Complex V or ATP synthase for the production of ATP from ADP. The number of molecules of ATP synthesis depends on the nature of electron donor. Oxidation of 1 molecule NADH results in 3 molecules of ATP. Figure given in the right shows the entire Electron Transport System in detail.

It is important to note that presence of oxygen is important for aerobic respiration, but its role is limited in the terminal stage of the process. Presence of oxygen is important because it drives the entire process by eliminating hydrogen from the process or it can be said that oxygen is the final hydrogen acceptor.

The Respiration Balance Sheet

Theoretically, we can calculate the net gain of ATP for every molecule of oxidized glucose and this calculation is based on following assumptions –

  • There is an orderly and sequential functioning of the pathway; with one substrate forming the next with glycolysis, Krebs cycle and Electron Transport System following one after another.

  • The NADH formed during glycolysis is transferred into mitochondria and oxidative phosphorylation takes place.

  • None of the intermediates in any process is utilized to synthesize any other compound.

  • No alternative substrates except glucose are respired.

All of the pathways work simultaneously but none of the above mentioned assumptions are really valid in living system. Substrate that enters the pathways are extracted as and when required, ATP is utilized as and when required, the rate of enzyme is controlled by several means. On the other hand, doing this exercise is important as it appreciate the efficiency and beauty of the living system in extracting and storing energy. Thus, there can be net gain of 36 ATP molecules from one molecule of glucose in case of aerobic respiration.

Following figures explains the net gain of ATP:

Net Gain of ATP


Amphibolic Pathway

The term amphibolic is used to explain “biological pathway that involves both catabolism and anabolism.”

Example of Amphibolic Pathway

Krebs cycle is an example of Amphibolic Pathway because it includes both catabolism of fatty acids and carbohydrates and synthesis of anabolic precursors for amino acid synthesis. Thus, the pathway with both catabolism and anabolism potential is known as amphibolic pathway.

Respiratory Quotient

This is another aspect of respiration. “Respiratory quotient is the ratio of CO2 produced to O2 consumed while food is being metabolized.”  

Where, RQ stands for Respiratory Quotient

RQ depends on the type of respiratory substrate used in respiration. When carbohydrate is used as substrate and is completely oxidized, RQ becomes 1. It implies equal amount of O2 and CO2 are consumed and evolved. This reaction is displayed in the figure below – 

In case, fats are used during the process of respiration, RQ becomes less than 1. Following equation shows the calculation for fatty acid and tripalmitin is used as substrate – 

When protein is used as respiratory substrates the ratio comes out to be 0.9.

Factors affecting Respiration in Plants

There are eight environmental factors that has significant impact on respiration in plants –

  • Oxygen content of the atmosphere

  • Effect of water content

  • Effect of temperature

  • Effect of availability of light

  • Impact of respirable material

  • Effect of concentration of carbon dioxide in atmosphere

  • Protoplasmic conditions, i.e. younger tissues have greater protoplasm as compared to older tissues.

  • Other factors, i.e. fluorides, cyanides, azides, etc.


Frequently Asked Questions (FAQs)

Q1. What is the metabolic fate of pyruvate?

Sol. It depends on the requirement of cell. There are three methods in which cell handle pyruvic acid produced via glycolysis process. These are alcoholic fermentation, aerobic respiration and lactic acid fermentation. The fermentation is carried out in anaerobic condition in case of unicellular eukaryotes and prokaryotes.


Q2. What is the difference between respiration and photosynthesis?




Respiration takes place in all living cells, including green (Chlorophylls) and non – green.

Photosynthesis takes place only in case of Chlorophylls cells.

It occurs both in night and day and includes Glycolysis – in cytoplasm, Krebs cycle/ Citric acid cycle – in mitochondrial matrix and Electron transport chain and oxidative phosphorylation in inner mitochondrial membrane.

Photosynthesis occurs only in light and includes Light reaction – in grana of chloroplast, Calvin cycle or dark reaction – in stroma of chloroplast and Photolysis or water splitting complex in thylakoid lumen

O2 is utilized and CO2 and H2O is formed

CO2 and water is used and O2 is released

It is a catabolic process which includes destruction of food.

It is an anabolic process which includes the manufactured food.

Oxygen is absorbed and carbohydrate is oxidized.

Oxygen is liberated and carbohydrate is synthesized.

It is an exothermic process and energy is released during respiration.

It is an endothermic process and energy is stored during the process.

Dry weight of plant decreases and potential energy is transformed into kinetic energy.

In this case, dry weight of plant increases and light energy is converted into potential energy.


Q3. What is cellular respiration?

Sol. “The biochemical process, which occurs within cells and oxidizes food to obtain energy, is called cellular respiration.” This process is catalyzed by several enzymes. Glycolysis is the first stage of cellular respiration.


Q4. What is external respiration in plants?

Sol. In plants, external respiration is carried out via simple diffusion. It takes place through –

  • Lenticels, i.e. opening in the bark of trunk.

  • Stomata present in leaves along with green part of stems.


Q5. Why during day, plants give out oxygen instead of taking it for respiration?

Sol. In plants, oxygen is released during the process of photosynthesis in day time. This oxygen is made available for respiration. Moreover, the rate of photosynthesis is much higher as compared to the rate of respiration. Thus, plants give out excessive oxygen in day time. Added to this, carbon dioxide is released during night because photosynthesis stops in the absence of sunlight.


Q6. Describe the significance of fermentation.

Sol. Fermentation process is very important in industries and micro – organisms, in very large number, are cultured and are used in following processes:

  • In bakeries, fermentation is used for preparing bread, biscuits and cakes.

  • In breweries, it is used in preparing several alcoholic drinks.

  • Fermentation is used in producing vinegar and in tanning and curing of leather.

  • In everyday life, this process is used in preparing curd, dhoklas, idlis, bhatura, etc. 


Q7. What is the difference between aerobic respiration and anaerobic respiration?


Aerobic Respiration

Anaerobic Respiration

In this respiration, oxygen is used.

Oxygen is not used in anaerobic respiration.

This respiration takes place in the cells of all higher animals and plants.

This process takes place in fungi, bacteria and several endoparasites.

Oxidation of glucose takes place completely.

Partial oxidation of glucose takes place.

The end product of this respiration is water and carbon dioxide.

The end product in this case is carbon dioxide and lactic acid or ethyl alcohol.

Oxidation of one molecule of glucose yields 38 ATP molecules.

Partial oxidation of one molecule of glucose yields 2 ATP molecules.

This process takes place in mitochondria and cytoplasm of cell.

This process takes place only in cytoplasm.


Q8. Write the equations of aerobic and anaerobic respiration.

Sol. Aerobic Respiration:

Glucose (C6 H12 O6) + 6O2 --------------------------------→ 6CO2 + 6H2O +38ATP

Anaerobic Respiration:

Glucose (C6 H12 O6) ----------------→ 2C2 H5 OH +2ATP (Alcohol fermentation)


Glucose (C6 H12 O6) -----------------→ 2CH3CHOHCOOH + 2ATP (Lactic acid fermentation)


Q9. What is the difference between breathing and respiration?




Breathing is the mechanism by which organisms obtain oxygen from air and release carbon dioxide. Thus, it includes expiration and inspiration.

It includes breathing and oxidation of food in the cells of the organism in order to release energy.

It is a physical process.

It is a biochemical and physical process

Breathing is carried out by lungs.

Respiration is carried out in lungs and mitochondria of cells.


Q10. What is the difference between Glycolysis and Krebs cycle?



Krebs Cycle

It is a linear pathway.

It is a cyclic pathway.

It occurs in cytoplasm of cell.

it occurs in the matrix of mitochondria.

It occurs in both aerobic and anaerobic respiration.

It occurs only in aerobic respiration.

It generates two NADH2 and two ATP molecules on the breakdown of one glucose molecule.

It generates 6 NADH2, 2FADH2 and 2 ATP molecules on the breakdown of 2 acetyl–CoA molecules.

Watch this Video for more reference


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